Research Project: Quantitative expression of liver enriched transcription factors in hepatocellular carcinoma, new markers for recurrence following curative treatment
Project Director: Iacob Razvan Andrei, MD, PhD
Mentor of The Project Director: Prof. Popescu Irinel, MD, PhD
Contractor: Fundeni Clinical Institute, Bucharest, Romania
Project duration: 2011-2013
Abstract:Hepatocellular carcinoma (HCC) is the fifth most common cancer worldwide. Due to the evolution of this neoplasm, the development of effective new systems that could predict the likelihood of recurrence after curative treatment, including molecular prognostic factors, is much needed. This will help in deciding therapeutic strategies for patients with HCC according to the predicted low or high risk of recurrence. New molecular insights of liver organogenesis have highlighted the paramount importance of the liver enriched transcription factors in generating and maintaining the differentiated liver specific phenotype: hepatic nuclear factor 1(HNF-1), HNF-3, HNF-4, HNF-6, CCAAT/enhancer binding protein (C/EBP), and D binding protein (DBP). The main objective of the proposed project is to investigate the expression level of liver enriched transcription factors in curatively treated hepatocellular carcinoma and the relationship with the differentiation degree of the tumor and the histopathological appearance. The results could lead to new molecular classifications of hepatocellular carcinoma with potential prognostic significance. The project has a significant translational clinical impact as prediction of early recurrence of HCC should lead to an aggressive follow-up after surgical therapy and could indicate the need for adjuvant chemotherapy. |
Project results:
Papers presented at National Meetings
Iacob R, Iacob S, Nastase A, Vagu C, Grigorie M, Dumitrascu T, Tanase AM, Dorobantu B, Stoica L, Paslaru L, Coriu D, Dima S, Croitoru A, Gheorghe C, Gheorghe L and Popescu I. Investigarea profilului expresiei genice a factorilor de transcriptie hepatici in carcinomul hepatocelular tratat cu viza curativa. Published Abstract in Journal of Gastrointestinal and Liver Diseases 2012. Paper presented at The XXXII’nd National Congress Of Gastroenterology, Hepatology And Digestive Endoscopy, Targu Mures 2012.
Iacob R, Vagu C, Nastase A, Iacob S, Grigorie M, Herlea V, Croitoru A, Dima S, Gheorghe C, Gheorghe L, Ott M, Popescu I. Expresia genica cantitativa a factorilor de transcriptie hepatici poate fi utilizata pentru predictia recurentei tumorale la pacientii cu hepatocarcinom tratati cu viza curativa. Published Abstract in Journal of Gastrointestinal and Liver Diseases 2013. Paper presented at The XXXIII’rd National Congress Of Gastroenterology, Hepatology And Digestive Endoscopy, Timisoara 2013.
Papers presented at International Meetings
Iacob R, Iacob S, Nastase A, Vagu C, Grigorie M, Dumitrascu T, Tanase AM, Dorobantu B, Stoica L, Paslaru L, Coriu D, Dima SO, Croitoru A, Gheorghe C, Gheorghe L, Popescu I. Quantitative gene expression of liver enriched transcription factors in curativelly treated hepatocellular carcinoma. Published abstract in Gut 2012. Paper presented at United European Gastroenterology Week 2012 – UEGW 2012, Amsterdam, The Netherlands.
Iacob R, Vagu C, Nastase A, Iacob S, Grigorie R, Herlea V, Croitoru A, Dima S, Gheorghe C, Gheorghe L, Ott M, Popescu I. Quantitative Gene Expression of Liver Enriched Transcription Factors Could Be Used to Predict Tumor Recurrence After Curative Treatment of Hepatocellular Carcinoma. Published Abstract in Gastroenterology 2013. Paper presented at Digestive Disease Week 2013, Orlando USA. Gastroenterology. Vol. 144, Issue 5, Supplement 1, Page S-1024.
Iacob R, Vagu C, Nastase A, Iacob S, Grigorie M, Stoica L, Herlea V, Croitoru A, Dima SO, Coriu D, Paslaru L, Gheorghe L, Gheorghe C, Ott M, Popescu I. Prediction of Hepatocellular Carcinoma Recurrence after Curative Treatment Using Quantitative Gene Expression of Liver Enriched Transcription Factors. Published Abstract in Hepatology International 2013. Paper presented at 23rd Conference of the Asian Pacific Association for the Study of the Liver – APASL 2013. Singapore.
Iacob R, Ruedrich U, Iacob S, Gheorghe C, Gheorghe L, Dima SO, Bock M, Ott M, Popescu I. Expression of Proteins Involved in Intercellular Junctions Is Strongly Correlated to Liver Enriched Transcription Factors in HUH7 Hepatoma Cells. Published Abstract in Hepatology International 2013. Paper presented at 23rd Conference of the Asian Pacific Association for the Study of the Liver – APASL 2013. Singapore.
Submitted articles
Razvan Iacob, Speranta Iacob, Liana Gheorghe, Cristian Gheorghe, Adina Croitoru, Vlad Herlea and Irinel Popescu. Outcome of liver transplantation for hepatocellular carcinoma – a single center experience. Original article submitted to Chirurgia 2013
Awards and distinctions:
1.Poster of distinction presentation during Digestive Disease Week at the Orange County Convention Center, in Orlando, FL, May 18-21, 2013 for the paper Quantitative gene expression of liver enriched transcription factors could be used to predict tumor recurrence after curative treatment of hepatocellular carcinoma.
2.APASL Liver Week Presidential Award 2013
3.First prize of the Romanian Society for Gastroenterology and Hepatology (SRGH) for the paper presented at the XXXIII’rd National Congress Of Gastroenterology, Hepatology And Digestive Endoscopy, Timisoara 2013: Expresia genica cantitativa a factorilor de transcriptie hepatici poate fi utilizata pentru predictia recurentei tumorale la pacientii cu hepatocarcinom tratati cu viza curativa
